Comparing the relative strength of mouse neoantigens using peptide MHC melting temperature.

Run your unknown peptide alongside two standards in the same differential scanning fluorimetry assay, and place it on a fixed 0–100 stability scale — comparable across labs, machines, and reagent lots.

All three Tm values should come from the same assay run.

Relative Strength scale

The two anchors from your run define a straight line: with SIINYEKL at 0 and SIINFEKL at 100. Every library antigen is placed on that line at its published strength, so the y-axis reads out the melting temperature you would expect for it in your run. Hover a small point to see it.

Low anchor · SIINYEKL High anchor · SIINFEKL Reference library Your antigen

Reference library

Below are the current reference points from the benchmarking paper, plus approved entries contributed by other labs.

AntigenPeptideStrengthSource