All three Tm values should come from the same assay run.
Relative Strength scale
The two anchors from your run define a straight line: with SIINYEKL at 0 and SIINFEKL at 100. Every library antigen is placed on that line at its published strength, so the y-axis reads out the melting temperature you would expect for it in your run. Hover a small point to see it.
Low anchor · SIINYEKL
High anchor · SIINFEKL
Reference library
Your antigen
Reference library
Below are the current reference points from the benchmarking paper, plus approved entries contributed by other labs.
| Antigen | Peptide | Strength | Source |
|---|